Salinity stress induces ionic and osmotic imbalances in rice plants that in turn negatively affect photosynthesis rate, resulting in growth retardation and yield penalty. Efforts have, therefore, been carried out to understand the mechanism of salt tolerance, however, the complexity of biological processes at proteome levels remains a major challenge. Here, we performed a comparative proteome and phosphoproteome profiling of microsome enriched fractions of salt-tolerant (cv. IR73; indica) and salt-susceptible (cv. Donjin; japonica) rice varieties. This approach led to the identification of 5,856 proteins, of which 473 and 484 proteins showed differential modulation between DJ and IR sample sets, respectively. The phosphoproteome analysis led to the identification of a total 10,873 phosphopeptides of these 2,929 and 3,049 significant phosphopeptides were identified in DJ and IR sample sets, respectively. The integration of proteome and phosphoproteome data showed activation of ABA and Ca2+ signaling components exclusively in IR in response to salt stress. Taken together, our results highlight the changes at proteome and phosphoproteome levels and provide a mechanistic understand of salt stress tolerance in rice.