In this project, the protein mixture in the adhesive strip samples was analyzed by means of intragel enzymatic hydrolysis and high-resolution biomass spectrometry. Among them, 10 ubiquitination modification sites were identified in the samples E1+E2+HERC5+ISG15+N, the target protein was identified in the samples E1+E2+HERC5+N and N, but the target modification was not identified on the target protein.