PXD053173

PXD053173 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to spermatozoa proteome
Description	The cryopreservation of avian semen without significant loss of fertilizing ability is of practical interest to the poultry industry and for the conservation of genetic biodiversity. However, the unique physiological features make avian spermatozoa highly sensitive to damage caused by cryopreservation. The potential proteomic changes in turkey (Meleagris gallopavo) semen throughout the cryopreservation process have never been investigated previously. Therefore, the purpose of the present study was to evaluate the proteomic changes of spermatozoa during cryopreservation at three stages (fresh, equilibrated, and frozen/thawed semen) using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF/TOF). No differentially expressed proteins (DEPs) were found in spermatozoa proteome of fresh and equilibrated semen. One hundred forty six protein spots differed significantly between fresh and frozen/thawed spermatozoa. One hundred three protein spots differed significantly between equilibrated and frozen/thawed spermatozoa. Most of the proteins that changed in response to cryopreservation were related to the generation of precursor metabolites and energy through aerobic respiration, flagellum-dependent cell motility, and binding of sperm to the zona pellucida. This suggests the complexity of the processes leading to decreases in the quality of cryopreserved semen.
HostingRepository	PRIDE
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-08_05:02:40.846.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariola Słowińska
SpeciesList	scientific name: Meleagris gallopavo (Common turkey); NCBI TaxID: 9103;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-17 08:33:47	ID requested
⏵ 1	2025-07-08 05:02:41	announced

10.1016/j.theriogenology.2025.117473;

Pardyak L, Liszewska E, Judycka S, Machci, ń, ska-Zieli, ń, ska S, Karol H, Dietrich MA, Goj, ł, o E, Arent Z, Bili, ń, ska B, Rusco G, Iaffaldano N, Ciereszko A, S, ł, owi, ń, ska M, The effect of cryopreservation on the turkey (Meleagris gallopavo) semen proteome. Theriogenology, 243():117473(2025) [pubmed]

submitter keyword: turkey, MALDI TOF/TOF, spermatozoa

Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.slowinska@pan.olsztyn.pl
lab head
Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.slowinska@pan.olsztyn.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD053173

PRIDE project URI

• PRIDE
  1. PXD053173
     1. Label: PRIDE project
     2. Name: Proteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to spermatozoa proteome