To elucidate the impact of GLV infection, we conducted a comparative analysis of the total protein profiles of tachyzoite samples. By analysing the mass spectrometry (MS) data from G. duodenalis DHGLV, using the G. duodenalis WBC6 isolate database as a reference, proteomics analyses revealed the actual protein species identified from the proteomics data were 1274 in the Giardia WBC6-GLV strain and 1494 in the Giardia DH+GLV strain.