PXD052987

PXD052987 is an original dataset announced via ProteomeXchange.

Title	Rhizobia T3E NopL interaction protein identified by LC-MSMS
Description	The coding sequence of the NopL was cloned into pET28a vector using BamH I and Sal I restriction enzymes. The resulting vector was transformed into E. coli BL21 (DE3) for the expression of recombinant protein. His-NopL protein was purified by Ni NTA Beads 6FF (Smart-lifesciences, SA005005). DN50 roots inoculated with HH103 were collected at 1 dpi and frozen in liquid nitrogen. Soybean root hair cells were collected by scraping the soybean roots using the cell scraper (Corning Incorporated, 3010). Soybean root hair proteins were extracted using protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% Glycerol, 0.2% Trixton X-100, 1 mM DTT, 1×protease inhibitor cocktail, 1 mM NaF, 1 mM Na3VO4 and 1 mM PMSF). The obtained His-NopL protein was added to the soybean root protein extract, while no His-NopL protein was added to the control sample. After mixing, the NopL interacting proteins were pull-down using Ni NTA Magarose Beads (Smart-lifesciences, SM008001) semi-in vivo. Finally, the eluted proteins were subjected to analysis using LC-MS/MS to identify and characterize the proteins that interact with NopL.
HostingRepository	iProX
AnnounceDate	2024-06-09
AnnouncementXML	Submission_2024-06-10_21:23:25.710.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xulun Dong
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-10 21:22:47	ID requested
⏵ 1	2024-06-10 21:23:26	announced

Dataset with its publication pending

submitter keyword: NopL, LC-MSMS

Qingshan Chen
contact affiliation	Northeast Agricultural University
contact email	1261640420@qq.com
lab head
Xulun Dong
contact affiliation	Northeast Agricultural University
contact email	otogishu0430@gmail.com
dataset submitter

iProX dataset URI