Activated eosinophils is a major cell type to be involved in allergic diseases. IL-5 primarily activates eosinophils and prolongs their survival. Notably, inflammatory cytokines including IL-33 and TNF-alpha are also important to establish and augment its inflammatory process in type 2 inflammation. The aim of this study is to clarify the role of these cytokines as direct activators for human eosinophils.