PXD052486

PXD052486 is an original dataset announced via ProteomeXchange.

Title	Loss of Eml1 alters microtubule-associated protein networks in mouse brain heterotopia
Description	The layered structure of the cerebral cortex is formed through a complicated sequence of highly controlled stages. During this process, the perturbations of neuronal migration and cell division can result in a rare disorder called cortical heterotopia. Heterotopia patients can have recurrent epileptic seizures, developmental delays, and mild intellectual disabilities. Studying heterotopia has been challenging because human mutations linked to the disease often do not result in heterotopia formation in mouse models. EML1 is a microtubule-binding protein, and it stands out as the first heterotopia-associated gene where mutations lead to heterotopia formation in both humans and mice. In this study, we conducted a comparative proteomic analysis of the Eml1 cKO heterotopic mice cortices and neuronal progenitor primary cells during cerebral cortex development. We performed label-free and dimethyl labeling-based quantitative proteomic approaches, and microtubule pelleting assays to understand how Eml1 depletion disrupts protein networks in cortical tissue and neuronal progenitor primary cells during cerebral cortex development.
HostingRepository	PRIDE
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-08_02:38:20.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Berfu Nur Yiğit
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-22 11:13:47	ID requested
⏵ 1	2025-07-08 02:38:21	announced

10.1038/S42003-025-08394-0;

submitter keyword: Cortical Development, Microtubules (MTs), Microtubule-associated proteins (MAPs), Cortical Malformation,Eml1, Proteomics

Nurhan Özlu
contact affiliation	Koc University, Department of Molecular Biology and Genetics, Istanbul, Turkiye
contact email	nozlu@ku.edu.tr
lab head
Berfu Nur Yiğit
contact affiliation	Koc University
contact email	byigit19@ku.edu.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD052486

PRIDE project URI

• PRIDE
  1. PXD052486
     1. Label: PRIDE project
     2. Name: Loss of Eml1 alters microtubule-associated protein networks in mouse brain heterotopia