PXD052434

PXD052434 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Binding factors of RNAPII isolated from the HeLa cell nuclei
Description	RNA polymerase II (RNAPII) is a major transcription protein that transcribes the genomic DNA packaged into chromatin. To reveal the RNAPII structures during transcription in the human genome, we extracted the RNAPII molecules inside the HeLa cell nuclei and determined the structures of transcribing RNAPII molecules associated with/without the transcription elongation factors and nucleosome. Our LC-MS/MS analysis revealed that the preinitiation complex components, such as Mediator, TFIIH, and TFIID, the elongation complex subunits, such as SPT4/5, SPT6, and ELOF1, and histones exist in the immunopurified fraction of RNAPII from HeLa cell nuclei.
HostingRepository	jPOST
AnnounceDate	2025-04-24
AnnouncementXML	Submission_2025-04-24_00:36:28.224.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lumi Negishi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-21 02:39:02	ID requested
⏵ 1	2025-04-24 00:36:28	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: RNAPII, transcription, nucleosome, histone, chromatin, nuclei

submitter keyword: RNAPII, transcription, nucleosome, histone, chromatin, nuclei

Contact List

Hitoshi Kurumizaka
lab head
Lumi Negishi
contact affiliation	The University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003120/