During infection and granuloma formation, pathogenic mycobacteria accumulate triacylglycerol in the form of Intrabacterial Lipid Inclusions (ILI). These organelles facilitate bacterial evasion from the immune system and serve as a carbon source to sustain bacterial persistence within the infected host., a rapidly-growing non-tuberculous opportunistic pathogen, was previously shown to generate similar structures throughout their infectious cycle. In this proteomics study, M. abscessus was used as a model organism to identify, at a global level, the ILI-associated proteins (IAP) involved in ILI accumulation.