PXD052245
PXD052245 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A Novel High-Throughput Immunoaffinity LC-MS/MS Assay for P III-NP and other Fragments of Type III Procollagen in Human Serum |
| Description | The amino-terminal propeptide of type III procollagen (P-III-NP) is used with IGF-I to detect the illicit use of growth hormone and to monitor growth hormone therapy. However, the only currently available assays for P-III-NP are immunoassays, which are not well harmonized. In addition, other fragments of type III procollagen may better evaluate collagen turnover. We developed a high-throughput assay using immunoaffinity enrichment coupled to ultra high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify peptides belonging to three different regions of type III procollagen in human serum simultaneously. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2024-10-28 |
| AnnouncementXML | Submission_2024-10-28_13:56:05.132.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Huu Hien Huynh |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Gln->pyro-Glu; Oxidation; Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4) |
| Instrument | Xevo TQ-S |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-05-13 22:30:40 | ID requested | |
| ⏵ 1 | 2024-10-28 13:56:05 | announced |
Publication List
| Huynh HH, Barahona-Carrillo L, Moncrieffe D, Cowan DA, Forrest K, Becker JO, Emrick MA, Thomas A, Thevis M, Eichner D, Byers PH, Miller GD, Hoofnagle AN, A Novel High-Throughput Immunoaffinity LC-MS/MS Assay for P-III-NP and Other Fragments of Type III Procollagen in Human Serum. Drug Test Anal, 17(8):1150-1159(2025) [pubmed] |
Keyword List
| submitter keyword: P-III-NP, collagen turnover, single-point calibrator |
Contact List
| Andy Hoofnagle | |
|---|---|
| contact affiliation | University of Washington |
| contact email | ahoof@uw.edu |
| lab head | |
| Huu Hien Huynh | |
| contact affiliation | University of Washington |
| contact email | hhuynh89@uw.edu |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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