PXD051909

PXD051909 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomics and proteomics analysis of LIM2405 Cell Line
Description	Non-invasive prognostic markers are needed to improve survival of colorectal cancer (CRC) patients. Towards this goal, we here apply integrative systems glycobiology approaches to tumour tissues and PBMCs from CRC patients and matching controls as well as a CRC patient-derived cell line. The untargeted -omics-driven approaches revealed that non-canonical paucimannosidic proteins from monocytic and cancer cell origins are prominent signatures in CRC tumour tissues, and that their expression associates with CRC progression. Guided by these novel relationships, we then show in vitro that N-acetyl-β-D-hexosaminidase (Hex) drives paucimannosidic protein biosynthesis in CRC cells, and is intimately involved in processes underpinning CRC metastasis (adhesion, migration, invasion). Importantly, Hex activity was elevated in PBMCs and plasma from patients with advanced CRC relative to those with early-stage disease. Notably, we show that plasma Hex activity accurately informs on CRC patient survival. Our study opens new avenues for effective prognostication and therapeutic intervention in CRC.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_15:39:19.279.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebeca Kawahara
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 19:19:47	ID requested
⏵ 1	2025-05-06 15:39:20	announced

10.1016/j.mcpro.2025.100927;

Kawahara R, Kautto L, Bansal N, Dipta P, Chau TH, Liquet-Weiland B, Ahn SB, Thaysen-Andersen M, HEXB Drives Raised Paucimannosylation in Colorectal Cancer and Stratifies Patient Risk. Mol Cell Proteomics, 24(3):100927(2025) [pubmed]

submitter keyword: LIM2405, proteomics, glycoproteomics,Colorectal Cancer

Morten Thaysen-Andersen
contact affiliation	1School of Natural Sciences, Macquarie University, Sydney, NSW, Australia 2Institute for Glyco-core Research (iGCORE), Nagoya University, Aichi, Japan
contact email	morten.andersen@mq.edu.au
lab head
Rebeca Kawahara
contact affiliation	Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia
contact email	rebecasakuma@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051909

PRIDE project URI

• PRIDE
  1. PXD051909
     1. Label: PRIDE project
     2. Name: Glycoproteomics and proteomics analysis of LIM2405 Cell Line