PXD051886

PXD051886 is an original dataset announced via ProteomeXchange.

Title	Condensin II Activation by the centromeric protein M18BP1
Description	Condensin I and condensin II promote drastic spatial compaction (condensation) of the genome upon mitotic entry. Condensin I binds chromatin only after dissolution of the nuclear envelope in prophase. Conversely, condensin II is nuclear throughout the cell cycle and initiates chromosome condensation. Previous studies demonstrated that MCPH1 inhibits condensin II to prevent chromosome condensation during interphase. Mechanisms that promote condensin II activation in early mitosis likely exist but are currently unknown. Through genetic and proteomic approaches, we identify M18BP1, a protein previously associated with centromere identity, as a factor required for condensin II localization to chromatin during mitosis. To activate and localize condensin II, M18BP1 directly binds the CAP-G2 subunit. M18BP1 and MCPH1 compete for CAP-G2 binding, and CDK1 phosphorylation promotes replacement of MCPH1 with M18BP1 to activate condensin II upon mitotic entry. Our results identify a fundamental and evolutionarily conserved mechanism of condensin II activation.
HostingRepository	PRIDE
AnnounceDate	2025-07-09
AnnouncementXML	Submission_2025-07-09_09:05:57.592.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051886
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Graziadei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 04:20:46	ID requested
⏵ 1	2025-07-09 09:05:58	announced

10.6019/PXD051886;

Borsellini A, Conti D, Cutts EE, Harris RJ, Walstein K, Graziadei A, Cecatiello V, Aarts TF, Xie R, Mazouzi A, Sen S, Hoencamp C, Pleuger R, Ghetti S, Oberste-Lehn L, Pan D, Bange T, Haarhuis JHI, Perrakis A, Brummelkamp TR, Rowland BD, Musacchio A, Vannini A, Condensin II activation by M18BP1. Mol Cell, ():(2025) [pubmed]

10.1016/j.molcel.2025.06.014;

submitter keyword: sda, sulfo-sda,condensin, cryo-em, xl-ms, m18bp1, crosslinking

Andrea Graziadei
contact affiliation	structural proteomics unit, national facility for structural biology, Human Technopole, Italy
contact email	andrea.graziadei@fht.org
lab head
Andrea Graziadei
contact affiliation	Human Technopole
contact email	andrea.graziadei@fht.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD051886

PRIDE project URI

• PRIDE
  1. PXD051886
     1. Label: PRIDE project
     2. Name: Condensin II Activation by the centromeric protein M18BP1