Updated project metadata. Humanin (HN) is a 24 amino acid peptide encoded by mitochondrial DNA MT-RNR2 (16S ribosomal RNA [rRNA]). It was previously shown, that HN protects tumor cells from damage during chemotherapy. To get mechanistic insight of HN induced singaling cascades involved in brain tumor growth, we performed a global phosphoproteomic analysis total and phosphorylated proteins on human brain tumor cells after treatment with the peptide Humanin.