The goal of this study was to identify the key functions of Candidatus Thiodiazotropha gloverae symbionts that are hosted in the gills of the lucinind clams, Lucinoma kazani, found at the deep-sea hydrocarbon seeps in the eastern Mediterranean Sea. These symbionts (Cromatiales, Sedimenticolaceae) are chemoautotrophs that use sulfide and thiosulfate as electron donors, and oxygen or nitrate as electron acceptors to fuel energy conservation. Yet, Ca. Thiodiazotropha gloverae ex. Lucinoma kazani can use alternative energy sources, such as methanol and formate. These symbionts encode the metabolic potential for dinitrogen fixation. In April 2021, we collected four L. kazani specimens near a brine pool in Palmahim Disturbance offshore Israel at a water depth of approximately 1150 m, (32° 13' 23.5" N 34° 10' 42.19" E), using a remotely-operated vehicle. They were fixed in RNAlater, which was discarded after 24 hours, and the specimens were kept at -80°C until DNA/RNA/protein co-extraction using the AllPrep DNA/RNA/Protein Mini Kit (Cat. No. 80004, Qiagen).