GalNAc-T2 was identified as a novel antiviral factor that promotes viral clearance and contributes to reduced disease severity.This project aimed to investigate the impact of GalNAc-T2 overexpression on proteomics and glycoproteomics in lung cell. We employ SILAC labeling, lectin-based affinity enrichment, and LC-ETD MS/MS to characterize the regulated proteins and glycosylation sites resulting from GalNAc-T2 overexpression.