PXD051019

PXD051019 is an original dataset announced via ProteomeXchange.

Title	Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications
Description	Livestock farming and conventional meat production pose significant environmental, health, and animal welfare challenges. In seeking sustainable alternative solutions, cultivated meat technology typically utilizes differentiation of myogenic progenitor cells (MPCs) into muscle cells for in vitro meat production. However, understanding the molecular determinants governing MPC differentiation into muscle cells, and the potential enhancement of this process through modulation of signaling pathways, remains limited. Herein, we characterized the molecular landscape associated with bovine MPC differentiation in vitro by employing multiomics, and explored its augmentation by small molecules, together leading to identification of media that enhanced myogenic differentiation compared with conventional methods in both 2D cultures and tissue‐engineered 3D skeletal muscle constructs. Through bulk and single‐cell transcriptomics and proteomics, we compared conventional and enhanced differentiation media, demonstrating that the enhanced media gave rise to unique progenitor‐like cell populations, while simultaneously promoting differentiation into myocytes and contractile myotubes expressing a wide array of myogenic markers that more closely resemble bovine muscle cells in vivo. The improved method for promoting myogenic differentiation in 2D and 3D formats, together with the corresponding molecular roadmap, may prove valuable for cultivated meat applications.
HostingRepository	PRIDE
AnnounceDate	2025-07-24
AnnouncementXML	Submission_2025-07-24_07:51:22.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ori Bar-Nur
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	methylated residue; mono N-acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-27 09:12:34	ID requested
⏵ 1	2025-07-24 07:51:22	announced

10.1002/ADVS.202413998;

submitter keyword: Multiomics,Cultivated Meat, Bovine

Ori Bar-Nur
contact affiliation	ETH Zürich, Department of Health Sciences and Technology, Institut of Human Movement Sc. and Sport, Laboratory of Regenerative and Movement Biology, Switzerland
contact email	ori.bar-nur@hest.ethz.ch
lab head
Ori Bar-Nur
contact affiliation	Department of Health Sciences and Technology, Institut of Human Movement Science and Sport, Laboratory of Regenerative and Muscle Biology
contact email	ori.bar-nur@hest.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD051019

PRIDE project URI

• PRIDE
  1. PXD051019
     1. Label: PRIDE project
     2. Name: Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications