Primary human umbilical vein endothelial cells (HUVECs, n=4) were exposed to low-magnitude pre-conditioning FSS (1.5 dynes/cm2) 18h using the Ibidi pump system followed by exposure to the arterial- (15 dynes/cm2) or venous-magnitude (1.5 dynes/cm2) FSS for 24h. Conditioned media was collected to analyse the effect of acute changes in the haemodynamic environment on the EC secretome.