Update information. LVTX-8, a peptide toxin synthesized from the cDNA library of the spider Lycosa vittata with 25 amino acids. In this study, we performed a series of cellular and molecular experiments such as cell toxicity, colony formation, and cell migration assays to exam the anticancer activity, selectivity and evaluate the cell toxicity of LVTX-8 in NPC cells (5-8F and CNE-2). The results suggested that LVTX-8 significantly inhibited cell proliferation and migration in both cell lines. To find the potential drug targets and further explain the molecular mechanisms underlying the anticancer capability of LVTX-8, high throughput proteomics profiling were performed.