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PXD050222 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNeuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding
DescriptionLearning and memory require activity-induced changes in dendritic translation, but which messenger RNAs (mRNAs) are involved and how they are regulated are unclear. Here, to monitor how depolarization impacts local dendritic biology, we employed a dendritically-targeted proximity labeling approach, followed by cross-linking immunoprecipitation (CLIP), ribosome profiling, and mass spectrometry. Depolarization of primary cortical neurons with KCl or the glutamate agonist DHPG causes rapid reprogramming of dendritic protein expression, where changes in dendritic mRNAs and proteins are weakly correlated. For a subset of pre-localized messages, depolarization increases translation of upstream open reading frames (uORFs) and their downstream coding sequences, enabling localized production of proteins involved in long term potentiation, cell signaling, and energy metabolism. This activity-dependent translation is accompanied by the phosphorylation and recruitment of the non-canonical translation initiation factor eIF4G2, and the translated uORFs are sufficient to confer depolarization-induced, eIF4G2-dependent translational control. These studies uncover an unanticipated mechanism by which activity-dependent uORF translational control by eIF4G2 couples activity to local dendritic remodeling.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEzgi Hacisuleyman
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-28 12:07:25ID requested
12024-04-02 08:27:30announced
Publication List
Keyword List
submitter keyword: neuron, RNA localization, localized translation
Contact List
Robert B Darnell
contact affiliationNeuro-oncology at the Rockefeller University
contact emaildarnelr@rockefeller.edu
lab head
Ezgi Hacisuleyman
contact affiliationScripps Research Institute
contact emailezgi.hacisuleyman@gmail.com
dataset submitter
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