In the prolonged arms race between viruses and their host plants, viruses usually encode viral suppressors of RNA silencing (VSR) to overcome the hosts' defense mechanism by inhibiting RNAi. Barley stripe mosaic virus (BSMV) is an RNA virus characterized by a positive single-stranded genetic structure, comprising three distinct genomic RNAs labeled as α, β, and γ. The multifunctional protein γb within BSMV’s RNAγ acts as a potent VSR, yet its underlying molecular mechanism requires further exploration. Previous studies on BSMV interacting proteins were conducted exclusively in yeast systems. We aim to identify the interacting proteins of γb under authentic conditions, specifically, the protein group interacting in the context of BSMV infection. The emerging proximity labeling (PL) technology offers several advantages, such as the identification of transiently interacting proteins, membrane proteins, and being non-toxic to plants. Consequently, this determines that PL technology may aid in resolving the challenge of identifying the interacting protein group of VSR in the context of BSMV infection.