PXD049325

PXD049325 is an original dataset announced via ProteomeXchange.

Title	Integrative single-plaque analysis reveals signature Aβ and lipid profiles in the Alzheimer’s brain
Description	Utilizing multimodal mass spectrometry imaging (MSI) combined with machine learning techniques, this study investigates the molecular heterogeneity of amyloid-β (Aβ) plaques and associated lipid profiles in post-mortem brain samples from Alzheimer’s disease (AD) and amyloid-positive cognitively unaffected (AP-CU) individuals. Our analytical approach permitted single-plaque level investigation, revealing distinct populations of amyloid plaques characterized by differential Aβ and lipid compositions. Notably, the integration of MSI data with machine learning based feature extraction enabled the identification of Aβ38 and ganglioside GM1 as significant molecular markers differentiating AD from AP-CU pathology. These findings suggest that the heterogeneity in Aβ metabolism and lipid homeostasis, as revealed through precise analysis, is a key factor in the pathogenesis of AD and implies that total amyloid burden alone is an insufficient marker for the disease. The application of MSI and machine learning based feature extraction in this context exemplifies a progressive analytic strategy to unravel complex biochemical phenomena, offering potential pathways for the refinement of diagnostic tools and deepening the understanding of neurodegenerative diseases from an analytical chemistry perspective.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:54.316.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Enzlein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF fleX; rapifleX

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-12 01:13:38	ID requested
1	2024-06-06 02:11:32	announced
⏵ 2	2024-10-22 06:43:54	announced	2024-10-22: Updated project metadata.

10.1021/ACS.ANALCHEM.3C05557;

submitter keyword: Alzheimer’s disease, Amyloid-β, MALDI MS imaging, Gangliosides, Machine learning

Carsten Hopf
contact affiliation	Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany. Mannheim Center for Translational Neuroscience (MCTN), Medical Faculty Mannheim, Heidelberg University, 69120 Heidelberg, Germany. Medical Faculty, Heidelberg University, 69120 Heidelberg, Germany.
contact email	c.hopf@hs-mannheim.de
lab head
Thomas Enzlein
contact affiliation	Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany.
contact email	t.enzlein@hs-mannheim.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD049325

PRIDE project URI

• PRIDE
  1. PXD049325
     1. Label: PRIDE project
     2. Name: Integrative single-plaque analysis reveals signature Aβ and lipid profiles in the Alzheimer’s brain