PXD049283

PXD049283 is an original dataset announced via ProteomeXchange.

Title	ct of PROTAC #89, which targets an endogenously SNAP-tagged clathrin light chain, on the HAP1 cell proteome
Description	We would like to investigate what effect degradation of the clathrin light chain has on the HAP1 proteome. We therefore tagged the clathrin light chain (CLC) in HAP1 cells endogenously with a SNAP-tag via CRISPaint. The degradation of the SNAP-tagged protein is induced by the addition of a SNAP-PROTAC (PROTAC #89). The proteomes of a short (6 h) and long (24 h) treatment with PROTAC #89 are compared against the corresponding DMSO controls using LFQ.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:16:52.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-08 08:56:49	ID requested
⏵ 1	2025-05-06 13:16:53	announced

10.1039/d4cb00184b;

Pol SA, Liljenberg S, Barr J, Simon G, Wong-Dilworth L, Paterson DL, Berishvili VP, Bottanelli F, Kaschani F, Kaiser M, Pettersson M, Hellerschmied D, Induced degradation of SNAP-fusion proteins. RSC Chem Biol, 5(12):1232-47(2024) [pubmed]

submitter keyword: PROTAC, clathrin, HAP1

Farnusch Kaschani
contact affiliation	Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD049283

PRIDE project URI

• PRIDE
  1. PXD049283
     1. Label: PRIDE project
     2. Name: ct of PROTAC #89, which targets an endogenously SNAP-tagged clathrin light chain, on the HAP1 cell proteome