To investigate the difference in protein expression between SCN1A-KO and WT cerebral organoids, quantitative proteomic analysis of SCN1A-KO and WT cerebral organoids (days 0 and 120) was carried out using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional nanoflow liquid chromatography-tandem mass spectrometry (2D-nLC-MS/MS)