Experimental group 1 and experimental group 2 were fed with shrimp feed containing 30 μ g/kg and 60 μ g/kg OMVs respectively, while the control group was not given any basic diet without any treatment. The feeding experiment of Penaeus vannamei was carried out in the indoor culture workshop of Guangdong Lanyin Aquatic Science and Technology Company in Wenchang City, Hainan Province. After the end of the domestication period, healthy Litopenaeus vannamei were randomly divided into 3 groups with 3 repeats in each group and 40 prawns in each group. The daily feeding amount is 5% of shrimp body weight and 8% of shrimp body weight, and the feeding amount is adjusted at any time according to the feeding situation of shrimp fry. Feed three times a day at 7:00, 14:00 and 20:00 every day, respectively. In order to keep the water environment of cultured shrimp stable, it was ventilated continuously for 24 hours during the experiment, and the temperature, salinity, pH value and ammonia nitrogen concentration were basically the same. Replace the original water body with new sand filtered seawater every 2 days, and remove unconsumed feed and fecal residues from each tank 2 hours after eating. Pay attention to the health status of prawns during the experiment. On the 28th day of the experimental cycle, 9 shrimps were randomly taken from each group, and the hepatopancreas were dissected and frozen in liquid nitrogen for 15 min, then immediately transferred to the refrigerator at -80 ℃ for preservation. Proteomic sequencing to probe the biological role of VpAHPND-OMVs.