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    <DatasetSummary announceDate="2024-02-19" hostingRepository="PRIDE" title="Structural basis of chloroplast transcription">
        <Description>Chloroplasts contain a dedicated genome, which encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. In this work, a 19-subunit PEP complex from Sinapis alba was purified by glycerol gradient centrifugation. All known subunits of the complex could be identified by LC-MS and the structure of the complex was determined by cryo-electron microscopy.</Description>
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        <Species>
            <cvParam cvRef="MS" accession="MS:1001469" name="taxonomy: scientific name" value="Sinapis alba"/>
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            <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Noah Ditz"/>
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            <cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="Department of plant proteomics,
Leibniz Univerity Hannover"/>
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            <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Hans-Peter Braun"/>
            <cvParam cvRef="MS" accession="MS:1000589" name="contact email" value="braun@genetik.uni-hannover.de"/>
            <cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="Department of Plant Proteomics, Leibniz University Hannover"/>
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