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PXD048930 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of N-Glycosylation for SARS-CoV-2 expressed receptor-binding domain VOCs and membrane protein with LC-MS.
DescriptionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect human cells by first attaching to the ACE-2 receptor via its receptor-binding domain (RBD) in the spike protein. We investigated the influence of N-glycosylation sites of the RBD and the membrane (M) protein on IgG antibody binding in serum samples from patients infected with the original SARS-CoV-2 strain in Germany. The N-glycosylation sites Asn331, Asn334, Asn343, Asn370 and Asn381 of RBDs of the wildtype, alpha, beta, gamma, kappa, and omicron BA.1 variants expressed in HEK293T and HEK293S GnTI- cells , as well as Asn5 for the M-protein were analyzed with tandem mass spectrometry for N-glycosylation.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDaniela Volke
SpeciesList scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationListDeamidated; Hex(5)HexNAc(2); Carbamidomethyl; HexNAc; Oxidation
InstrumentSYNAPT G2-Si
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-26 17:24:25ID requested
12024-03-26 22:51:50announced
22024-04-29 14:21:07announced: Added PubMed Id
Publication List
Schwarze M, Volke D, Rojas Echeverri JC, Schick R, Lakowa N, Gr, ü, newald T, Wolf J, Borte S, Scholz M, Krizsan A, Hoffmann R, Influence of Mutations and N-Glycosylation Sites in the Receptor-Binding Domain (RBD) and the Membrane Protein of SARS-CoV-2 Variants of Concern on Antibody Binding in ELISA. Biology (Basel), 13(4):(2024) [pubmed]
Keyword List
submitter keyword: SARS-CoV-2, N-Glycosylation, HD-DDA, ESI-IMS-Q-TOF-MS
Contact List
Ralf Hoffmann
contact affiliationUniversität Leipzig/BBZ
contact emailralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliationUniversität Leipzig/BBZ
contact emaildaniela.volke@bbz.uni-leipzig.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI