Uranium is a non-essential and toxic metal for plants. Uranium accumulates preferentially in the roots and the bulk of the radionuclide is associated with the insoluble proteome. To elucidate new molecular mechanisms related to U stress response and tolerance in Arabidopsis thaliana, the aim of the project is the use of label-free quantitative proteomics to analyze the dynamics of the root membrane- and cell wall-enriched proteome under uranium stress.