PXD048771

PXD048771 is an original dataset announced via ProteomeXchange.

Title	Synaptic vesicle proteomics with age and in synucleinopathies
Description	Neurotransmitter release occurs through exocytosis of synaptic vesicles. α-Synuclein’s function and dysfunction in Parkinson’s disease and other synucleinopathies is tightly linked to synaptic vesicle binding. Age is the biggest risk factor for synucleinopathy, and ~15% of synaptic vesicle proteins are linked to diseases of the central nervous system. Yet, age- and disease-induced changes in synaptic vesicles remain unexplored. For an unbiased analysis of the synaptic vesicle proteome, we applied quantitative mass spectrometry to crude synaptic vesicles isolated at three time points (1 month, 3 months, and 10 months of age) from wild-type mice, alpha-synuclein knockout mice, and alpha-synuclein BAC mice, which express human wild-type alpha-synuclein under all human regulatory elements in absence of mouse alpha-synuclein, providing human-like spatiotemporal expression of alpha-synuclein and recapitulating motor and pathological features of Parkinson’s disease. We identified specific changes in synaptic vesicle proteins that capture aging and synucleinopathies. These findings not only provide new insights into synaptic vesicle biology and mechanisms of synucleinopathy, but also offer a baseline for further mechanistic exploration of age- and disease-related alterations in synaptic vesicles.
HostingRepository	PRIDE
AnnounceDate	2025-05-02
AnnouncementXML	Submission_2025-05-02_04:48:44.733.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacqueline Burré
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-22 12:09:05	ID requested
⏵ 1	2025-05-02 04:48:45	announced

10.1038/S41467-025-59441-7;

submitter keyword: synuclein,Synaptic vesicle, mouse, age

Jacqueline Burré
contact affiliation	Appel Alzheimer's Disease Research Institute & Brain and Mind Research Institute Weill Cornell Medicine New York, NY USA
contact email	jab2058@med.cornell.edu
lab head
Jacqueline Burré
contact affiliation	Weill Cornell Medicine
contact email	jab2058@med.cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048771

PRIDE project URI

• PRIDE
  1. PXD048771
     1. Label: PRIDE project
     2. Name: Synaptic vesicle proteomics with age and in synucleinopathies