Entomopathogenic nematodes, the beneficial soil-dwelling nematodes identified with noteworthy ecological services of insect pest management, naturally, harbor and vector obligate bacterial symbionts which together cause rapid insect mortality. The tritrophism among the bacterium-nematode-insect host is a treasure box of biological, molecular and biochemical information that was examined to a limited extent in other studies. We have, herein, aimed at quantifying the concentration and at generating the total proteomic profiles of one insect host, Spodoptera frungiperda, during the progression of infection with 4 EPN and their associated bacteria, as compared to that of healthy controls. The time-course expression of larval proteins of the test insect, Spodoptera frugiperda, during the progression of larval mortality due to nematode-bacterium infection, more specifically,4 different combinations of Heterorhabditis indica-Photorhabdus luminescens, Steinernema spp. vs Xenorhabdus spp., respectively. The proteomic profiling was facilitated through LC-MS/MS. The data submitted relates to the insect protein profiles (LC-MSMS) in healthy, infected and morbid, and dead larvae of Spodoptera frugiperda.