Myc-ERF34 was expressed and precipitated using the Co-Immunoprecipitation in HEK293T Cells method. Seven-day-old Col4 seedlings were incubated in liquid 1/2 MS medium supplemented with 100 μM ABA for 3 hours. Subsequently, they were lysed using NEBT lysis buffer [20mM HEPES pH 7.5, 40mM KCl, 1mM EDTA, 1% TritonX-100] containing 1× EDTA-free Protease Inhibitor Cocktail Tablets. The resulting lysate supernatant was incubated with the precipitated Myc-ERF34. Beads were washed three times with NEBT wash buffer [20mM HEPES pH 7.5, 40mM KCl, 1mM EDTA, 0.01% TritonX-100], and then 30 µL of 4× loading buffer was added, followed by incubation at 95°C for 10 minutes