Updated project metadata. The soluble proteome of Thermochaetoides thermophila (T. thermophilia) was analyzed to identify possible interaction of coeluting complexes for downstream integrative analysis using Co-fractionation/mass spectrometry (CF/MS). Mycelium of T. thermophilia was lysed and separated via size exclusion chromatography (SEC) and the Fractions were probed for their protein composition using LC-MSMS. Label-free Quantification was utilized to determine intra- and inter-fraction protein abundance, deriving information regarding, stochiometry and metabolic complex organization.