PXD048337-1

PXD048337 is an original dataset announced via ProteomeXchange.

Title	Domains architecture and phospho-switches of eIF3e translation subunit control pollen tube growth and morphology
Description	The eIF3e subunit form an octameric ribonucleoprotein complex to initiate translation, and its PCI domain engage in the assembly of the proteolytic 26S proteosome supercomplex. These two complexes are central in translation initiation and protein quality surveillance and turnover. However, how eIF3e is structurally regulated remain unknown. Here, using RIPseq approach we revealed that eIF3e co-immunoprecipitate with mRNAs involved in RNA biosynthesis (U3 snRNA12a and ncRNA processing) and cell morphology, and its PCI domain is crucial for regulation of pollen tube pulse growth and eIF3e dissociation post initiation. We show that motifs within eIF3e associated target RNAs can repress or activate translation. Since the PCI domain associate with the proteosome machinery, we show that pharmacological inhibition of the proteosome complex accumulate eIF3e, eIF3eΔPCI and the proteosome lid subunits RPN7/RPN12a to the nucleus and generate nuclear condensates. Importantly, constitutive dephosphorylation of eIF3e revealed antagonistic phosphosites at the PCI domain that balance pollen tube growth and cellular membrane morphology. These findings inform structural features that control eIF3e activities and that antagonistic de-phosphorylation of eIF3e maintain equilibrium of pollen tube growth rate dynamics and membrane structural morphology.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:15:43.517.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petr Pompach
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: NEWT:4097;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-09 03:22:10	ID requested
⏵ 1	2026-04-05 16:15:44	announced

10.1093/plcell/koag005;

Kumar V, Merret R, Carpentier MC, Honys D, Hafidh S, Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis-elements to regulatepollen tube growth. Plant Cell, 38(2):(2026) [pubmed]

submitter keyword: eIF3, Proteosome, Ribosome profiling,Translation initiation, Polarity, RIPseq, Pollen tube, PCI domain, Phosphorylation

Said Hafidh
contact affiliation	Laboratory of Pollen Biology, Lab 105 Institute of Experimental Botany (IEB ASCR) Czech Republic
contact email	hafidh@ueb.cas.cz
lab head
Petr Pompach
contact affiliation	Intitute of Biotechnology
contact email	petr.pompach@ibt.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD048337

PRIDE project URI

• PRIDE
  1. PXD048337
     1. Label: PRIDE project
     2. Name: Domains architecture and phospho-switches of eIF3e translation subunit control pollen tube growth and morphology