Entomopathogenic nematodes, the beneficial soil-dwelling nematodes identified with noteworthy ecological services of insect pest management naturally, harbor and vector obligate bacterial symbionts which together cause rapid insect mortality. The tritrophism among the bacterium-nematode-insect host is a treasure box of biological, molecular and biochemical information that was examined to a limited extent. In this project, we intend to explore the time-course expression of proteins of test insect, Holotrichia serrata larvae, during the progression of larval mortality due to nematode-bacterium infection, more specifically, Heterorhabditis indica- Photorhabdus luminescens, Steinernema spp. vs Xenorhabdus spp., respectively. The data submitted relates to the insect protein profiles (LC-MSMS) in healthy, infected and morbid, and dead larvae of Holotrichia serrata.