PXD048280

PXD048280 is an original dataset announced via ProteomeXchange.

Title	Visualization of the Cdc48 AAA+ ATPase protein unfolding pathway: Substrate Identification
Description	The Cdc48 AAA+ ATPase is an abundant and essential enzyme that unfolds substrates in multiple protein quality control pathways. The enzyme includes two conserved AAA+ ATPase cassettes, D1 and D2, that assemble as hexameric rings with D1 stacked above D2. Here, we report an ensemble of structures of Cdc48 affinity purified from lysate in complex with the adaptor Shp1 in the act of unfolding substrate. Our analysis reveals a continuum of structural snapshots that spans the entire translocation cycle. These data reveal new elements of Shp1-Cdc48 binding and support a “hand-over-hand” mechanism in which the sequential movement of individual subunits is closely coordinated. D1 hydrolyzes ATP and disengages from substrate prior to D2, while D2 rebinds ATP and re-engages with substrate prior to D1, thereby explaining the dominant role played by D2 in substrate translocation/unfolding.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:09:06.757.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Price
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-05 09:33:08	ID requested
⏵ 1	2024-10-17 05:09:07	announced

Cooney I, Schubert HL, Cedeno K, Fisher ON, Carson R, Price JC, Hill CP, Shen PS, Visualization of the Cdc48 AAA+ ATPase protein unfolding pathway. Nat Commun, 15(1):7505(2024) [pubmed]

10.1038/s41467-024-51835-3;

submitter keyword: Human, SHP1 pulldown

John C. Price
contact affiliation	Brigham Young University
contact email	drjohncprice@gmail.com
lab head
John Price
contact affiliation	Brigham Young University
contact email	drjohncprice@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048280
     1. Label: PRIDE project
     2. Name: Visualization of the Cdc48 AAA+ ATPase protein unfolding pathway: Substrate Identification