The prohibition of feeding processed animal proteins (PAPs) has been relaxed gradually since 2007. The official control method for PAPs in feedingstuff, a combination of light microscopy (LM) followed by PCR is no longer sufficient. Thus, a targeted LC-MS/MS method was developed, which enables a tissue specific distinction between PAPs derived from blood, muscle, skin (gelatine), egg and dairy products of the species ruminants, pig, poultry and fish. Tissue specific proteins were analyzed after reduction, alkylation and tryptic digestion to peptides by data dependent acquisition (DDA) and data independent acquisition (DIA) with high-resolution ESI-QTOF-MS. A targeted method was developed based on untargeted proteomics approaches and selection of specific peptides in accordance with generally applicable criteria. IIdentification of unique peptides for all authorized and non-authorized animal products of the four species was successful. The established method with a total of 45 unique peptides is the first which matches all requirements for feed control due the relaxing of feed ban for PAPs. Proficiency testing of blank and spiked samples revealed excellent results for trueness and selectivity; no false positive or negative results were observed. Furthermore, sensitivity was achieved to a level of 0.1 % (w/w) of the spiked samples that fulfills the EU requirements. Summing up, the developed method seems to be suitable for routine analysis and the performance should be evaluated by further ring trials.