Updated project metadata. We studied the effect of Ser30 with or without O-GlcNAc modification on K18 protein and its co-immunoprecipitated proteins in cholangiocarcinoma cells.The lysates from HuCCT1 shK18 stable cell line transfected with FLAG-K18-WT or FLAG-K18-S30A were captured with anti-FLAG beads, and then subjected to in-gel trypsin digestion and LC-MS/MS analysis.