Sweet potato (Ipomoea batatas), a starchy root and tuber crop, is an important source of carbohydrates for the human diet and a valuable resource in many industrial applications. Here, we performed a spatiotemporally distributed proteome analysis using leaves and storage roots of two F1 individuals, H283 (high starch) and L423 (low starch), of similar high yield to underly regulatory networks governing starch accumulation.