Quantitative label-free proteomic analysis of extracellular vesicles.
A pool of isolated proteins from extracellular vesicles (EVs) was performed for each sample group, namely healthy participants (HP), liver cirrhosis (LC), and hepatocellular carcinoma (HCC), split into three biological replicates. Then, samples were digested with trypsin, and the peptides were subjected to Nano LC-MS/MS Analysis and Mass Spectrometry. Nine raw MS files were analyzed and searched against the human protein database based on the species of the samples using Maxquant. The parameters were set as follows: the protein modifications were carbamidomethylation (C) (fixed), oxidation (M) (variable); the enzyme specificity was set to trypsin; the maximum missed cleavage was set to 2; the precursor ion mass tolerance was set to 10 ppm, and MS/MS tolerance was 0.5 Da.