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PXD047389

PXD047389 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabel-free quantitative proteomics analysis of ASPC-1 and H358
DescriptionA total of one million AsPC-1 and H358 cells were treated with DMSO or 100 nM MCB-36 for 12 hours, after which the cells were collected by centrifugation and stored at -80°C until further analysis. Proteins were reduced, alkylated, and purified by chloroform/meth anol extraction prior to trypsin digestion. The search parameters included tryptic digestion with up to two missed cleavages allowed, carbamidomethylation (C) as a fixed modification, and oxidation (M) and acetylation (protein N-terminal) as variable modifications. Peptide identification was carried out with an initial precursor ion mass tolerance of 10 ppm and a fragment mass tolerance of 0.02 Da.
HostingRepositoryiProX
AnnounceDate2023-11-30
AnnouncementXMLSubmission_2025-07-03_18:45:38.739.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhengke Lian
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-29 21:15:38ID requested
12024-02-22 18:32:05announced
22025-07-03 18:45:39announced2025-07-04: Update information
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: KRAS, PROTAC
Contact List
Xiufeng Pang
contact affiliationEast China Normal University
contact emailxfpang@bio.ecnu.edu.cn
lab head
Zhengke Lian
contact affiliationEast China Normal University
contact email625906916@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI