PXD047389
PXD047389 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label-free quantitative proteomics analysis of ASPC-1 and H358 |
Description | A total of one million AsPC-1 and H358 cells were treated with DMSO or 100 nM MCB-36 for 12 hours, after which the cells were collected by centrifugation and stored at -80°C until further analysis. Proteins were reduced, alkylated, and purified by chloroform/meth anol extraction prior to trypsin digestion. The search parameters included tryptic digestion with up to two missed cleavages allowed, carbamidomethylation (C) as a fixed modification, and oxidation (M) and acetylation (protein N-terminal) as variable modifications. Peptide identification was carried out with an initial precursor ion mass tolerance of 10 ppm and a fragment mass tolerance of 0.02 Da. |
HostingRepository | iProX |
AnnounceDate | 2023-11-30 |
AnnouncementXML | Submission_2025-07-03_18:45:38.739.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhengke Lian |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-11-29 21:15:38 | ID requested | |
1 | 2024-02-22 18:32:05 | announced | |
⏵ 2 | 2025-07-03 18:45:39 | announced | 2025-07-04: Update information |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: KRAS, PROTAC |
Contact List
Xiufeng Pang | |
---|---|
contact affiliation | East China Normal University |
contact email | xfpang@bio.ecnu.edu.cn |
lab head | |
Zhengke Lian | |
contact affiliation | East China Normal University |
contact email | 625906916@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |