PXD047268 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Probing the trypanosome nuclear pore by TurboID proximity labelling |
Description | Previous BioID experiments targeting the nucleoporin (NUP) NUP158 and the nuclear transport factor MEX67 indicated (PMID: 36410438; PXD031245) that proximity labelling delivers highly specific interactome data in the confined localisation of the nuclear pore, with a labelling radius well below the size of the nuclear pore. Here we extended this approach to target NUPs NUP110, NUP76 and NUP96 with a TurboID-HA tandem tag fused to both, the N and C-terminus of each respective target protein. While the central NUP96 biotinylated most nuclear pore proteins with the marked exception of the nuclear basket, NUP158, NUP110 and NUP76 caused biotinylation of specific sub-complexes of the pore and Mex67 labelled NUPs lining the pore channel. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-04 |
AnnouncementXML | Submission_2023-12-04_03:58:06.521.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Zoltner |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-27 04:01:47 | ID requested | |
⏵ 1 | 2023-12-04 03:58:07 | announced | |
Publication List
Moreira CMDN, Kelemen CD, Obado SO, Zahedifard F, Zhang N, Holetz FB, Gauglitz L, Dallagiovanna B, Field MC, Kramer S, Zoltner M, Impact of inherent biases built into proteomic techniques: Proximity labeling and affinity capture compared. J Biol Chem, 299(1):102726(2023) [pubmed] |
10.1016/j.jbc.2022.102726; |
Keyword List
submitter keyword: BioID, Trypanosoma brucei, porin, nuclear transport, nuclear pore |
Contact List
Dr. Martin Zoltner |
contact affiliation | Head of Drug Discovery and Evaluation Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic phone: +420 325 873 966 |
contact email | zoltnerm@natur.cuni.cz |
lab head | |
Martin Zoltner |
contact affiliation | Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH |
contact email | m.zoltner@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD047268 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047268
- Label: PRIDE project
- Name: Probing the trypanosome nuclear pore by TurboID proximity labelling