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PXD047268

PXD047268 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProbing the trypanosome nuclear pore by TurboID proximity labelling
DescriptionPrevious BioID experiments targeting the nucleoporin (NUP) NUP158 and the nuclear transport factor MEX67 indicated (PMID: 36410438; PXD031245) that proximity labelling delivers highly specific interactome data in the confined localisation of the nuclear pore, with a labelling radius well below the size of the nuclear pore. Here we extended this approach to target NUPs NUP110, NUP76 and NUP96 with a TurboID-HA tandem tag fused to both, the N and C-terminus of each respective target protein. While the central NUP96 biotinylated most nuclear pore proteins with the marked exception of the nuclear basket, NUP158, NUP110 and NUP76 caused biotinylation of specific sub-complexes of the pore and Mex67 labelled NUPs lining the pore channel.
HostingRepositoryPRIDE
AnnounceDate2023-12-04
AnnouncementXMLSubmission_2023-12-04_03:58:06.521.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartin Zoltner
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-27 04:01:47ID requested
12023-12-04 03:58:07announced
Publication List
Moreira CMDN, Kelemen CD, Obado SO, Zahedifard F, Zhang N, Holetz FB, Gauglitz L, Dallagiovanna B, Field MC, Kramer S, Zoltner M, Impact of inherent biases built into proteomic techniques: Proximity labeling and affinity capture compared. J Biol Chem, 299(1):102726(2023) [pubmed]
10.1016/j.jbc.2022.102726;
Keyword List
submitter keyword: BioID, Trypanosoma brucei, porin, nuclear transport, nuclear pore
Contact List
Dr. Martin Zoltner
contact affiliationHead of Drug Discovery and Evaluation Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic phone: +420 325 873 966
contact emailzoltnerm@natur.cuni.cz
lab head
Martin Zoltner
contact affiliationDivision of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact emailm.zoltner@dundee.ac.uk
dataset submitter
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Dataset FTP location
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