PXD046897

PXD046897 is an original dataset announced via ProteomeXchange.

Title	Type III-B CRISPR-Cas signaling-based cascade of proteolytic cleavages
Description	Characteristic for type III CRISPR-Cas systems is the generation of cyclic oligoadenylate (cOA) molecules, which allosterically activate proteins carrying cognate sensory domains: CARF or SAVED. Here, we characterize a type III-B system associated set of genes from Haliangium ochraceum, containing two CBASS co-opted caspase-like proteases, SAVED-CHAT and PCaspase (Prokaryotic Caspase). Cyclic tri-AMP (cA3)- induced oligomerization of the SAVED domains of SAVED-CHAT leads to proteolytic activity of the CHAT domains which, when active, specifically cleave and activate PCaspase. In turn, activated PCaspase cleaves a multitude of proteins and leads to a strong defence mechanism in vivo in E. coli.. Together, our findings show a cascade of proteolytic activities (with conceptual similarities to eukaryotic caspases) and constitute an effective strategy to defend against mobile genetic elements.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:06:06.919.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Scheltema
SpeciesList	scientific name: Haliangium ochraceum DSM 14365; NCBI TaxID: 502025;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 05:09:36	ID requested
⏵ 1	2025-05-06 12:06:07	announced

10.1126/science.adk0378;

Steens JA, Bravo JPK, Salazar CRP, Yildiz C, Amieiro AM, K, ö, stlbacher S, Prinsen SHP, Andres AS, Patinios C, Bardis A, Barendregt A, Scheltema RA, Ettema TJG, van der Oost J, Taylor DW, Staals RHJ, Type III-B CRISPR-Cas cascade of proteolytic cleavages. Science, 383(6682):512-519(2024) [pubmed]

submitter keyword: Type III CRISPR-Cas

Richard Scheltema
contact affiliation	Utrecht University
contact email	r.a.scheltema@uu.nl
lab head
Richard Scheltema
contact affiliation	Utrecht University
contact email	r.a.scheltema@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD046897

PRIDE project URI

• PRIDE
  1. PXD046897
     1. Label: PRIDE project
     2. Name: Type III-B CRISPR-Cas signaling-based cascade of proteolytic cleavages