Mytilus coruscus were injected with microbes into the adductor muscle. The microbes are Staphylococcus aureus, Vibrio alginolyticus, and Candida albicans. After microbial challenge, hemolymph was collected from the adductor muscle of M. coruscus at 2h post bacterial challenge. The hemolymph was isolated by 12% SDS-PAGE and the differential bands were collected for LC-MS/MS proteomic analysis.