PXD046669

PXD046669 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Simultaneous Proteome Localization and Turnover Analysis, MS3 Data
Description	The functionality of proteins is dependent on their spatial and temporal distributions, neither of which is directly measured by static protein abundance. Here we report a mass spectrometry-based proteomics workflow and data analysis pipeline, named Simultaneous Proteome Localization and Turnover (SPLAT), to concurrently examine the turnover dynamics and subcellular distributions of whole cell proteomes under perturbation. SPLAT builds on prior work in protein turnover measurements and subcellular localization profiling, by combining dynamic stable isotope labeling, differential ultracentrifugation, and kinetic modeling to concurrently measure changes in protein turnover and subcellular localization under perturbation in one experiment. Briefly, dynamic SILAC labeled cell lysates were fractionated with ultracentrifugation, digested using a modified FASP protocol, and multiplexed with TMT-10 plex tags. The pooled sample was then fractionated with RPLC and injected into an Orbitrap Fusion Tribrid mass spectrometer coupled to an LC with electrospray ionization source operated in data dependent acquisition mode. Detailed methods can be found in the submission files.
HostingRepository	jPOST
AnnounceDate	2023-11-08
AnnouncementXML	Submission_2023-11-08_12:20:36.230.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Robinson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-04 19:03:59	ID requested
⏵ 1	2023-11-08 12:20:36	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: MS3, AC16, spatial proteomics, spatiotemporal, protein turnover, dynamic SILAC, TMT

submitter keyword: MS3, AC16, spatial proteomics, spatiotemporal, protein turnover, dynamic SILAC, TMT

Contact List

Dr. Edward Lau
lab head
Sean Robinson
contact affiliation	University of Colorado, Anschutz Medical Campus
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002377/