Water is one of the resources most affected by the intensification of human activities. As consequences of these activities, we have eutrophication and global warming, which can also favor the blooms of cyanobacteria that produce cyanotoxins. Here we present the hepatic proteome of females and males of catfish Rhamdia quelen exposed to four experimental conditions, for 96 hours: filtered water at 25°C (F2, F3 and F4 as females; F17, F19 and F20 as males), filtered water at 30°C (F5, F6 and F7 as females; F22, F23 and F24 as males), extract equivalent to 100,000 cells/mL of Raphidiopsis raciborskii dissolved in filtered water at 25°C (F9, F10 and F11 as females; F25, F26 and F28 as males); and extract of 100000 cells/mL of Raphidiopsis raciborskii dissolved in filtered water at 30°C (F13, F14 and F15 as females; F29, F30 and F32 as males).