Updated project metadata. Unlike most bacteria characterized to date at the level of proteins involved in cell division, Salmonella has two alternative penicillin-binding proteins (PBP3 and PBP3SAL) that can direct peptidoglycan synthesis independently to build a septum that separates daughter cells. PBP3SAL main feature is an activity restricted to acidic pH conditions. In this study, we have pulled-down the divisome complex in bacteria lacking either PBP3 or PBP3SAL and identified the proteins that are components of the complex.