PXD046163

PXD046163 is an original dataset announced via ProteomeXchange.

Title	Evidence for an RNAi-independent role of DICER-LIKE2 in conferring growth inhibition and basal antiviral resistance
Description	Higher plants encode four or five DICER-LIKE (DCL) enzymes responsible for the production of small non-coding RNAs which function in RNA interference (RNAi). Different RNAi pathways in plants effect transposon silencing, antiviral defense and endogenous gene regulation. DCL2 acts genetically redundantly with DCL4 to confer basal antiviral defense, but in other settings, DCL2 has the opposite function of DCL4, at least in formal genetic terms. For example, knockout of DCL4 causes growth defects that are suppressed by inactivation of DCL2. Current models maintain that the biochemical basis of both of these effects is RNAi via DCL2-dependent small interfering RNAs (siRNAs). Here, we report that neither DCL2-mediated antiviral resistance nor growth defects can be explained by silencing effects of DCL2-dependent siRNAs. Both functions are defective in genetic backgrounds that maintain high levels of DCL2-dependent siRNAs, either through specific point mutations in DCL2 or simply by reducing DCL2 dosage in plants heterozygous for dcl2 knockout alleles. Intriguingly, however, all functions of DCL2 depend on it having some level of catalytic activity. We discuss this requirement for catalytic activity, but not for the resulting siRNAs, in the light of recent findings that reveal a function of DCL2 in activation of innate immunity in response to cytoplasmic double-stranded RNA.
HostingRepository	PanoramaPublic
AnnounceDate	2024-07-26
AnnouncementXML	Submission_2024-07-26_15:31:55.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jakob Woessmann
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-16 09:50:03	ID requested
⏵ 1	2024-07-26 15:31:56	announced

Nielsen CPS, Arribas-Hern, á, ndez L, Han L, Reichel M, Woessmann J, Daucke R, Bressendorff S, L, ó, pez-M, á, rquez D, Andersen SU, Pumplin N, Schoof EM, Brodersen P, Evidence for an RNAi-independent role of Arabidopsis DICER-LIKE2 in growth inhibition and basal antiviral resistance. Plant Cell, 36(6):2289-2309(2024) [pubmed]

submitter keyword: DCL2

Erwin Schoof
contact affiliation	Technical University of Denmark
contact email	erws@dtu.dk
lab head
Jakob Woessmann
contact affiliation	Technical University of Denmark
contact email	jakowo@dtu.dk
dataset submitter

Panorama Public dataset URI