5mL PAO1 culture was mixed with PaoP5(dap1 mutant) or PaoP5 (MOI=10) for 10 min, and 5mL PAO1(lon mutant) was infected with aoP5(dap1 mutant) (MOI=10) for 10 min. The 1ml sample was then made into pellets. proteomic analysis was performed by Applied Protein Technologies. Biological replication tests were performed 3 times in each group. The samples were lysed with SDT buffer and quantified with BCA protein assay kit. The protein is digested with trypsin and then desalted with a C18 cartridge. The digested peptides were concentrated by vacuum centrifuge, recombined with formic acid, and then analyzed by LC-MS/MS. The peptides were uploaded to a reversed-phase column and connected to a C18 reversed-phase analysis column of formic acid, separated by a linear gradient buffer containing acetonitrile and formic acid at a flow rate of 300 nL/min. The mass spectrometer operates in positive ion mode. The original MS data of each sample were combined, and MaxQuant 1.5.3.17 software was used for identification and quant