The whole blood samples were obtained from 3 LTNPs and 2 RPs HIV-1 infected patients, and the CD4+ T cells were isolated using anti-CD4-specific antibody-coated microbeads (130-045-101, Miltenyi Biotec, Germany) according to the manufacturer's instructions. Then the proteins extracted from the CD4+ T cells were lysed and labeled with Tandem Mass Tag (TMT), followed by quantified using synchronous precursor selection (SPS)-MS3 technology.