Our data support that following optic nerve crush injury, ocular infiltration of immune cells causes damage to the retinal vasculature and compromises the integrity of the blood-retina barrier (BRB). Functional ablation of integrin alphaM (CD11b) attenuates immune cell trafficking, protects the BRB, and strongly enhances β-glucan-elicited retinal ganglion cells axon regeneration. Here, we performed an unbiased analysis of the vitreal proteome; we harvested vitreous humor from WT and Itgam-/- mice, β-glucan treated after optic nerve injury, and used mass spectrometry-based proteomics for differential analysis of the WT and Itgam-/- vitreous proteome (label free and tandem mass tagging).