Extracellular vesicles (EVs) are heterogeneous particles encapsulated with a phospholipid bilayer membrane. EVs have evolved diverse biological functions, serving mainly as prominent mediators and regulators of cell-cell communication. Fungal EVs contain numerous factors involved in a diverse array of biological functions including biogenesis of extracellular matrix proteins and saccharides, antifungal resistance, and pathogenesis. This study investigated whether candidalysin, a peptide toxin that drives C. albicans infections, is present in EVs derived from C. albicans biofilms. We found that those EVs contain candidalysin at low levels and can permeabilize planar lipid bilayers membranes in a dose-dependent manner but are unable to damage oral epithelial cells (OECs). Application of EVs to OECs induced cytokine responses but did not activate MAPK signalling. Notably, EVs obtained from biofilms cultured for 24 h and 48 h exhibited differences in cargo composition and their ability to activate OECs. This study suggests that secreted EVs can serve as a toxin delivery system during C. albicans infection but also possess different functions depending on their maturity.